sendai virus sev strain 52 Search Results


2×105 pfu sev  (ATCC)
95
ATCC 2×105 pfu sev
2×105 Pfu Sev, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2×105 pfu sev/product/ATCC
Average 95 stars, based on 1 article reviews
2×105 pfu sev - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
sendai virus strain 52 (sev-52  (Charles River Laboratories)
90
Charles River Laboratories sendai virus strain 52 (sev-52
A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of <t>SeV</t> <t>52</t> per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.
Sendai Virus Strain 52 (Sev 52, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sendai virus strain 52 (sev-52/product/Charles River Laboratories
Average 90 stars, based on 1 article reviews
sendai virus strain 52 (sev-52 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
bca protein assay bca kit  (EuroClone)
90
EuroClone bca protein assay bca kit
A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of <t>SeV</t> <t>52</t> per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.
Bca Protein Assay Bca Kit, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bca protein assay bca kit/product/EuroClone
Average 90 stars, based on 1 article reviews
bca protein assay bca kit - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
sev 52 vr-105  (Charles River Laboratories)
90
Charles River Laboratories sev 52 vr-105
A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of <t>SeV</t> <t>52</t> per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.
Sev 52 Vr 105, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sev 52 vr-105/product/Charles River Laboratories
Average 90 stars, based on 1 article reviews
sev 52 vr-105 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
sendai virus (sev) strain sendai/52  (Fushimi Pharmaceutical)
90
Fushimi Pharmaceutical sendai virus (sev) strain sendai/52
KEY RESOURCES TABLE
Sendai Virus (Sev) Strain Sendai/52, supplied by Fushimi Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sendai virus (sev) strain sendai/52/product/Fushimi Pharmaceutical
Average 90 stars, based on 1 article reviews
sendai virus (sev) strain sendai/52 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
pseva6be ng s plasmid 155267  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier
pseva2be s plasmid 155268  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier
h-pdcawleaqqedsev-oh  (Biosynth Carbosynth)
N/A
   Buy from Supplier
h-dvlgtgafsevilaedk-oh  (Biosynth Carbosynth)
N/A
   Buy from Supplier
quantitative genomic rna from severe acute respiratory syndrome-related coronavirus 2 strain usa/md-hp05285/2021  (ATCC)
N/A
Genomic RNA isolated from a preparation of Severe acute respiratory syndrome-related coronavirus 2 isolate USA/MD-HP05285/2021. This product can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and
   Buy from Supplier
pseva6be ye1 s plasmid 155269  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier
pseva6be s plasmid 155266  (Addgene inc)
N/A
Standard format Plasmid sent in bacteria as agar stab
   Buy from Supplier

Image Search Results


A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of SeV 52 per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.

Journal: bioRxiv

Article Title: Murine Parainfluenza Virus Persists in Lung Innate Immune Cells Sustaining Chronic Lung Pathology

doi: 10.1101/2023.11.07.566103

Figure Lengend Snippet: A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of SeV 52 per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.

Article Snippet: Sendai virus strain 52 (SeV-52) stocks were expanded in 10-days-old embryonated chicken eggs (Charles River Laboratories, Wilmington, MA) and virus titers were determined using end-point dilution tissue culture infectious dose (TCID 50 ) infectivity assays in LLC-MK2 cells.

Techniques: Saline, Infection, RNA Expression, Virus, Quantitation Assay, Staining, Immunofluorescence, Microscopy

KEY RESOURCES TABLE

Journal: Cell

Article Title: Divergent sensory pathways of sneezing and coughing

doi: 10.1016/j.cell.2024.08.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Sendai virus (SeV) strain Sendai/52 Fushimi , ATCC , VR-105.

Techniques: Virus, Plasmid Preparation, Recombinant, Labeling, Digital PCR, Avidin-Biotin Assay, Lysis, Reverse Transcription, RNAscope, Multiplex Assay, Mutagenesis, TaqMan Assay, Software, Real-time Polymerase Chain Reaction